Research achievements


Spatially Organized Enzymes Drive Cofactor-Coupled Cascade Reactions.
T. A. Ngo, E. Nakata, M. Saimura, T. Morii
J. Am. Chem. Soc. 2016, 138, 3012-3021.

Latent pH-responsive ratiometric fluorescent cluster based on self-assembled photoactivated SNARF derivatives.
E. Nakata, Y. Yukimachi, Y. Uto, H. Hori, T. Morii
Sci. Tech. Adv. Mater. 2016, 17, 431-436.


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Validating subcellular thermal changes revealed by fluorescent thermosensors.
S. Kiyonaka, R. Sakaguchi, I. Hamachi, T. Morii, T. Yoshizaki, Y. Mori
Nature Methods 2015, 12, 801-802.


A modular zinc finger adaptor accelerates the covalent linkage of proteins at specific locations on DNA nanoscaffolds.
E. Nakata, H. Dinh, T. A. Ngo, M. Saimura, T. Morii,
Chem. Commun. 2015, 51, 1016-1019.

Self-Assembled Fluorescent Nanoprobe for the Detection of Fluoride Ions in Aqueous Solutions.
E. Nakata, Y. Nazumi, Y. Yukimachi, Y. Uto, H. Hori, T. Morii,
Bull. Chem. Soc. Jpn., 2015, 88, 327-329.


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Phosphorylation regulates fibrillation of an aggregation core peptide in the second repeat of microtubule-binding domain of human tau.
Inoue, M.; Kaida, S.; Nakano, S.; Annoni, C.; Nakata, E.; Konno, T.; Morii, T.
Bioorg. Med. Chem. 2014, 22, 6471-6480.


Peptidosteroid Tweezers Revisited: DNA Binding Through an Optimised Design.
Carrette, L. L., Morii, T., Madder, A.
Eur. J. Org. Chem. 2014, 2883-2891.


A protein adaptor to locate a functional protein dimer on molecular switchboard
Ngo, T. A.; Nakata, E.; Saimura, M.; Kodaki, T.; Morii, T.
Methods 2014, 67, 142-150.


A novel strategy to design latent ratiometric fluorescent pH probes based on self-assembled SNARF derivatives
Nakata, E.; Yukimachi, Y.; Nazumi, Y.; Uwate, M.; Maseda, H.; Uto, Y.; Hashimoto,T.; Okamoto, Y.; Hori, H.; Morii, T.
RSC Advances 2014, 4, 348-357.


和光純薬時報  2014, 82, 6-8


ナノ学会会報  2014, 12, 51-56


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Toxicity inspired cross-linking for probing DNA-peptide interactions.
Carrette, L. L., Morii, T., Madder, A.
Bioconjug. Chem. 2013, 24, 2008-2014.


Genetically encoded fluorescent thermosensors visualize subcellular thermoregulation in living cells.
Kiyonaka, S., Kajimoto, T. Sakaguchi, R., Shinmi, D., Omatsu-Kanbe, M., Matsuura, H., Imamura, H., Yoshizaki, T., Hamachi, I., Morii, T., Mori, Y.
Nat. Methods 2013, 10, 1232-1238.


Simultaneous Detection of ATP and GTP by Covalently Linked Fluorescent Ribonucleopeptide Sensors.
Nakano, S.; Fukuda, M.; Tamura, T.; Sakaguchi, R.; Nakata, E.; Morii, T.
J. Am. Chem. Soc. 2013, 135, 3465-3473.


A Peptide Nucleic Acid (PNA) Heteroduplex Probe Containing an Inosine-Cytosine Base Pair Discriminates a Single-Nucleotide Difference in RNA.
Matsumoto, K.; Nakata, E.; Tamura, T.; Saito, I.; Aizawa, Y.; Morii, T.
Chem. Eur. J. 2013, 19, 5034-5040.


Arranging enzymes and receptors with nanometer-scale precision on molecular switchboards
Ngo A. T.; Nakata, E.; Morii, T.
J. Biomol. Struct. Dyn. 2013, 31, 86


Recent progress in the construction methodology of fluorescent biosensors based on biomolecules
Nakata, E.; Liew, F.F.; Nakano, S.; Morii, T.
"Biosensors for Health, Environment and Biosecurity / Book 1", ISBN 978-953-307-155-8


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Construction of ratiometric fluorescent sensors by ribonucleopeptides.
Annoni, C.; Nakata, E.; Tamura, T.; Liew, F. F.; Nakano, S.; Gelmi, M. L.; Morii, T.
Org. Biomol. Chem. 2012, 10, 8767-8769.


Zinc finger proteins for site-specific protein positioning on DNA origami.
Nakata, E.; Liew, F. F.; Uwatoko, C. Kiyonaka, S. Mori, Y.; Katsuda, Y.; Endo, M.; Sugiyama, H.;
Morii T.
Angew Chem. Int. Ed. 2012, 51, 2421-2424.


Positional effects of phosphorylation on the stability and the morphology of tau-related amyloid fibrils.
Inoue, M.; Konno, T.; Tainaka, K.; Nakata, E.; Yoshida, H.; Morii, T.
Biochemistry 2012, 51, 1396-1406.


Study on the Application of Furan Crosslinking at the Protein-DNA Interface
Carrette, L. L. G.; Gattner, M.; Van den Begin, J.; Carell, T.; Morii, T.; Madder, A.
J. Pep. Sci. 2012, 18, S99.


中田 栄司, 森井 孝
日本化学会 編著, 東京, 2012年11月


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Design and synthesis of biotinylated inositol 1,3,4,5-tetrakisphosphate targeting Grp1 pleckstrin homology domain. Anraku, K.; Inoue, T.; Sugimoto, K.; Kudo, K.; Okamoto, Y.; Morii, T.; Mori, Y.; Otsuka, M. Bioorg. Med. Chem., 2011, 19, 6833-6841.


A ribonucleopeptide module for effective conversion of an RNA aptamer to a fluorescent sensor. Liew, F. F.; Hayashi, H.; Nakano, S.; Nakata, E.; Morii, T. Bioorg. Med. Chem., 2011, 19, 5771-5775.


Construction of dopamine sensors by using fluorescent ribonucleopeptide complexes. Liew, F. F.; Hasegawa, T.; Fukuda, M.; Nakata, E.; Morii, T. Bioorg. Med. Chem., 2011, 19, 4473-4481.


Facile conversion of RNA Aptamers to modular fluorescent sensors with tunable detection wavelengths. Nakano, S., Nakata, E., Morii, T. Bioorg. Med. Chem. Lett., 2011, 21, 4503-4506.


Structural Aspects for the Recognition of ATP by Ribonucleopeptide Receptors. Nakano, S., Mashima, T., Matsugami, A., Inoue, M., Katahira, M., Morii, T. J. Am. Chem. Soc., 2011, 133, 4567-4579.

Design and synthesis of highly solvatochromic fluorescent 2'-deoxyguanosine and 2'-deoxyadenosine analogs. Matsumoto K, Takahashi N, Suzuki A, Morii T, Saito Y, Saito I. Bioorg Med Chem Lett, 2011, 21, 1092-1099.

Selective assembly of photosynthetic antenna proteins into a domain-structured lipid bilayer for the construction of artificial photosynthetic antenna systems: structural analysis of the assembly using surface plasmon resonance and atomic force microscopy. Sumino A, Dewa T, Kondo M, Morii T, Hashimoto H, Gardiner AT, Cogdell RJ, Nango M. Langmuir, 2011, 27, 1092-1099.

The mechanism of fibril formation of a non-inhibitory serpin ovalbumin revealed by the identification of amyloidogenic core regions. Tanaka N, Morimoto Y, Noguchi Y, Tada T, Waku T, Kunugi S, Morii T, Lee YF, Konno T, Takahashi N. J. Biol. Chem. 2011, ,286, 5584-5594.

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Design of environmentally sensitive fluorescent 2'-deoxyguanosine containing arylethynyl moieties: Distinction of thymine base by base-discriminating fluorescent (BDF) probe. Y. Shinohara, K. Matsumoto, K. Kugenuma, T. Morii, Y. Saito, I. Saito. Bioorg. Med. Chem. Lett. 2010, 20, 2817-2820.

An in vitro fluorescent sensor reveals intracellular Ins(1,3,4,5)P4 dynamics in single cells. Sakaguchi, R., Tainaka, K., Shimada, N., Nakano, S., Inoue, M., Kiyonaka, S., Mori, Y., Morii, T. Angew. Chem. Int. Ed. 2010, 49, 2150-2153.

Design Strategies of Fluorescent Biosensors Based on Biological macromolecular Receptors. Tainaka, K., Sakaguchi, R., Hayashi, H., Nakano, S., Liew, F.-F., Morii, T. Sensors, 2010, 10, 1355-1376.

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Selective and direct inhibition of TRPC3 channels underlies biological activities of a pyrazole compound. Kiyonaka S, Kato K, Nishida M, Mio K, Numaga T, Sawaguchi Y, Yoshida T, Wakamori M, Mori E, Numata T, Ishii M, Takemoto H, Ojida A, Watanabe K, Uemura A, Kurose H, Morii T, Kobayashi T, Sato Y, Sato C, Hamachi I, Mori Y. Proc Natl Acad Sci U S A. 2009 Mar 31;106(13):5400-5. Epub 2009 Mar 16.

The amyloid fibrillization of phosphorylated human tau core peptides. Inoue, M., Tainaka, K., Hirata, A., Konno, T., Morii, T. Trans. Mat. Res. Soc. Jpn. 2009, 34, 517-520.

Development of A Fluorescent Ribonucleopeptide Sensor for Histamine. Fukuda, M., Hayashi, H., Hasegawa, T., Morii, T. Trans. Mat. Res. Soc. Jpn. 2009, 34, 515-527.

A single circularly permutated GFP sensor for inositol-1,3,4,5-tetrakisphosphate based on a split PH domain. Sakaguchi, R., Endoh, T., Yamamoto, S., Tainaka, K., Sugimoto, K., Fujieda, N., Kiyonaka, S., Mori, Y., Morii, T. Bioorg. Med. Chem. 2009, 17, 7381-7386.

Covalently linked fluorescent ribonucleopeptide sensors. Fukuda, M., Fong-Fong, L., Morii, T. Nucleic Acids Symp Ser. 2009, 53, 257-8.

Structural aspects for the function of ATP-binding ribonucleopeptide receptors. Nakano, S., Fukuda, M., Mashima, T., Katahira, M., Morii, T. Nucleic Acids Symp Ser. 2009, 53, 259-60.

Structural analysis of ribonucleopeptide aptamer against ATP. Mashima, T., Matsugami, A., Nakano, S., Inoue, M., Fukuda, M., Morii, T., Katahira, M. Nucleic Acids Symp Ser. 2009, 53, 267-8.

A Modular Strategy for Development of RNA-Based Fluorescent Sensors. Fukuda, M., Hasegawa, T., Hayashi, H., Morii, T. "Combinatorial methods for chemical and biological sensors." Chapter 10, pp. 249-269, R. A. Potyrailo and V. M. Mirsky (Eds.), Springer, 2009.

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Design and synthesis of biotinylated inositol phosphates relevant to the biotin-avidin techniques. Anraku, K., Inoue, T., Sugimoto, K., Morii, T., Mori, Y., Okamoto, Y. and Otsuka, M. Org. Biomol. Chem. 6, 1822-1830 (2008).

Context-dependent fluorescence detection of a phosphorylated tyrosine residue by a ribonucleopeptide. Hasegawa, T., Hagiwara, M., Fukuda, M., Nakano, S., Fujieda, N., and Morii, T. J. Am. Chem. Soc. 130, 8804-8812 (2008).

Charge-pairing mechanism of phosphorylation effect upon amyloid fibrillation of human tau core peptide. Inoue M, Hirata A, Tainaka K, Morii T, Konno T. Biochemistry. 2008 Nov 11;47(45):11847-57. Epub 2008 Oct 16.

Construction of a stable functional ribonucleopeptide complex by the covalent linking method. Fukuda M, Nakano S, Tainaka K, Fujieda N, Morii T. Nucleic Acids Symp Ser (Oxf). 2008;(52):195-6.

Selective recognition of a tetra-amino-acid motif containing phosphorylated tyrosine residue by ribonucleopeptide. Nakano S, Hasegawa T, Fukuda M, Fujieda N, Tainaka K, Morii T. Nucleic Acids Symp Ser (Oxf). 2008;(52):199-200.

Development of ribonucleopeptide-based fluorescent sensors for biologically active amines based on the stepwise molding strategy. Tainaka K, Hasegawa T, Fukuda M, Nakano S, Fujieda N, Morii T. Nucleic Acids Symp Ser (Oxf). 2008;(52):201-2.

Design of extremely facile 3'- and 5'-ends free molecular beacons using C8 alkylamino substituted 2'-deoxyguanosine. Matsumoto, K., Shinohara, Y., Numajiri, K., Ishioroshi, S., Morii, T., Saito, Y., Saito, I Nucleic Acids Symp Ser. 2009, 53, 141-2.

細胞内シグナル伝達物質リアルタイム検出用プローブ 森井 孝 「バイオインダストリー」、Vol. 25 (2), 7-14, (2008).

RNA-タンパク質複合体の分子進化を利用したアプタマーとセンサーの開発 福田将虎、森井 孝 生物物理、Vol. 48, 239-242 (2008)

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Synergic action of polyanionic and non-polar cofactors in fibrillation of human islet amyloid polypeptide. T. Konno, S. Oiki and T. Morii FEBS Lett. 581, 1635-1638 (2007).

Amyloid-forming propensity of the hydrophobic non-natural amino acid on the fibril-forming core peptide of human tau. Hirata, A., Sugimoto, K., Konno, T., Morii, T. Bioorg. Med. Chem. Lett. 17, 2971-4 (2007).

Stepwise functionalization of ribonucleopeptides: optimization of the response of fluorescent ribonucleopeptide sensors for ATP. T. Hasegawa, M. Hagihara, M. Fukuda, T. Morii Nucleosides Nucleotides & Nucleic Acids 26, 1277-1281 (2007).

Structure-based design of fluorescent biosensors from ribonucleopeptide complexes. H. Hayashi, M. Inoue, T. Morii Nucleic Acids Symp. Ser. 51, 95-96, (2007).

Controlling a substrate-binding geometry of ribonucleopeptide receptor. M. Fukuda, S. Nakano, T. Morii Nucleic Acids Symp. Ser. 51, 421-422 (2007).

Construction of ribonucleopeptide-based fluorescent sensors for biologically active amines. T. Hasegawa, H. Hayashi, T. Morii Nucleic Acids Symp. Ser. 51, 423-424 (2007).

Design of Ribonucleopeptide-based Receptors and Fluorescent Sensors. T. Morii

J. Biomol. Struct. Dynam. 24, 689, (2007).

RNA-ペプチド複合体をもとにした機能性分子の創製 森井 孝 「ゲノム化学:医学、分子生物学への応用と展開」化学フロンティア18, pp. 45-52,(2007)

テーラーメイド蛍光センサーの開発 森井 孝 「化学工業、Vol. 58 (5), 38-43, (2007).

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Nitric oxide activates TRP channels by cysteine S-nitrosylation. Yoshida T, Inoue R, Morii T, Takahashi N, Yamamoto S, Hara Y, Tominaga M, Shimizu S, Sato Y & Mori Y. Nature Chem. Biol. 2, 596-607 (2006).

A modular strategy for tailoring fluorescent biosensors from ribonucleopeptide complexes. M. Hagihara, M. Fukuda, T. Hasegawa, and T. Morii J. Am. Chem. Soc .128, 12932-12940 (2006).

ケミカルバイオロジーのツールとしての機能性ミニチュアタンパク質  森井 孝  化学工業、Vol. 57 (9), 8(656)-12(660), (2006).

「ファージディスプレイ法を用いたセレクション (標的分子に結合する生体分子の作製)」福田将虎、杉本健二、森井 孝 生命科学研究レター, 22, 24-29 (2006)

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Stepwise Molding of a Highly Selective Ribonucleopeptide Receptor. S. Sato, M. Fukuda, M. Hagihara, Y. Tanabe, K. Ohkubo and T. Morii J. Am. Chem. Soc .127, 30-31 (2005).

A Ribonucleopeptide Receptor Targets Phosphotyrosine. T. Hasegawa, M. K. Ohkubo, S. yoshikawa and T. Morii J. Surf. Sci. Nanotech .3, 41-45 (2005)

Covalent blocking of amyloid formation and protein aggregation by transglutaminase-catalyzed intra-molecular cross-linking. Konno, T. Morii, A. Hirata, S. Sato, H. Naiki, S. Oiki, and K. Ikura Biochemistry 44, 2072-2079 (2005).

Dual DNA recognition codes of a short peptide derived from the basic leucine zipper protein EmBP1. A. Hirata, M. Ueno, Y. Aizawa, K. Ohkubo, T. Morii, S. Yoshikawa Bioorg. Med. Chem .13, 3107-16 (2005).

Paradoxical inhibition of protein aggregation and precipitation by transglutaminase-catalyzed intermolecular cross-linking. T. Konno, T. Morii, H. Shimidzu, S. Oiki, K. Ikura J. Biol. Chem. 280, 17520-5 (2005).

Ribonucleopeptides recognize the phosphotyrosine residue. T. Hasegawa, S. Yoshikawa, T. Morii Nucleic Acids Res. Symp. Ser. 49, 79-80 (2005).

Stepwise functionalization of ribonucleopeptide complexes to receptors and sensors. M. Fukuda, Y. Tanabe, T. Morii Nucleic Acids Res. Symp. Ser. 49, 355-356 (2005).

敬虔な生体高分子ファン? それとも・・・  森井 孝  RNA Network Newsletter, volume 3, number 2, 59-62, January 2005.

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Ribonucleopeptide receptors. T, Morii, K. Ohkubo and S. Yoshikawa Biopolymers 71, 11 (2004)

Ribonucleopeptides: functional RNA-peptide complexes. M. Hagihara, T. Hasegawa, S. Sato, S. Yoshikawa, K. Ohkubo and T. Morii Biopolymers 76, 66-68 (2004)

Novel real time sensors to quantitatively assess in vivo inositol 1,4,5-trisphosphate production in intact cells. K. Sugimoto, M. Nishida, M. Otsuka, K. Makino, K. Ohkubo, Yasuo Mori and T. Morii Chem. Biol. 11, 475-485 (2004).

Design of sensing ribonucleopeptides for small ligands. M. Hagihara, T. Hasegawa, Y. Tanabe, S. Sato, S. Yoshikawa, K. Ohkubo, T. Morii Nucleic Acids Res. Symp. Ser. 48, 33-34 (2004).

Recent advances in biosensing second messengers. K. Sugimoto, R. Sakaguchi, Y. Mori and T. Morii Recent Res. Devel. Chem, 2, 73-89 (2004)

タンパク質・核酸をもとにした機能性分子設計 森井 孝 生命化学研究レター No.16, 10-18, 2004年10月

Ribonucleopeptide receptors. M. Hagihara, T. Hasegawa, S. Sato, S. Yoshikawa, K. Ohkubo, T. Morii Peptide Revolution: Genomics, Proteomics & Therapeutics. M. Chorev, p. 556-558, T. K. Sawyer, Ed., American Peptide Society (2004).

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Functional Reassembly of a Split PH Domain. K. Sugimoto, Y. Mori, K. Makino, K. Ohkubo and T. Morii J. Am. Chem. Soc. 125, 5000-5004 (2003).

Regulatory interaction of sodium channel IQ-motif with calmodulin C-terminal lobe. M. Mori, T. Konno, T. Morii, K. Nagayama, and K. Imoto Biochem. Biophys. Res. Commun. 307, 290-296 (2003).

Amplification of receptor signalling by Ca2+ entry-mediated translocation and activation of PLCg2 in B lymphocytes. N. Nishida, K. Sugimoto, Y. Hara, E. Mori, T. Morii, T. Kurosaki and Y. Mori EMBO J .22, 4677-4688 (2003).

Design of a ribonucleopeptide biosensor. M. Hagihara, A. Hirata, K. Ohkubo and T. Morii Nucleic Acids Res. Suppl., 3, 193-194 (2003).

Optimization of an ATP-binding ribonucleopeptide receptor. S. Sato, T. Hasegawa, M. Hagihara, M. Fukuda, K. Ohkubo and T. Morii Nucleic Acids Res. Suppl., 3, 239-240 (2003).

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Formation of a Fairly Stable Diazoate Intermediate of 5-Methyl-2'-deoxycytidine by HNO2 and NO, and Its Implication to a Novel Mutation Mechanism in CpG Site. T. Suzuki, M. Yamada, T. Nakamura, H. Ide, K. Kanaori, K. Tajima, T. Morii, and K. Makino Bioorg. Med. Chem .10, 1063-1067 (2002).

A General Strategy to Determine a Target DNA Sequence of Short Peptide: Application to a D-Peptide. T. Morii, T. Tanaka, S. Sato, M. Hagihara, Y. Aizawa and K. Makino J. Am. Chem. Soc. 124, 180-181 (2002).

A New Fluorescent Biosensor for Inositol Trisphosphate. T. Morii, K. Sugimoto, K. Makino, M. Otsuka, K. Imoto, and Y. Mori J. Am. Chem. Soc. 124, 1139-1140 (2002).

Structure-Based Design of a Leucine Zipper Protein with New DNA Contacting Region. T. Morii, S. Sato, M. Hagihara, Y. Mori, K. Imoto and K. Makino Biochemistry 41, 2177-2183 (2002).

In Vitro Selection of ATP-binding Receptors Using a Ribonucleopeptide Complex. T. Morii, M. Hagihara, S. Sato and K. Makino J. Am. Chem. Soc. 124, 4617-4622 (2002).

Chemical Approach to Untangle the Sequence-Specific DNA Binding by Proteins. S. Sato, M. Hagihara, K. Sugimoto and T. Morii Chem-Eur. J. 22, 5067-5071 (2002).

A new strategy to determine the target DNA sequence of a short peptide. S. Sato, H. Honjyo, S. Tsuji, M. Hagihara, K. Makino and T. Morii Nucleic Acids Res. Suppl., 2, 205-206 (2002).

Sequence-specific DNA binding by short peptides. T. Morii and K. Makino Advances in DNA Sequence Specific Agents, volume 4, 105-137 (2002).

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